By Herbert Begemann
-Enhanced sixth version includes extra illustrations and documented figures
-A ideal reference for hematological and oncological departments and the clincal labortory
-Classified findings from the realm overall healthiness Organization
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Extra resources for Atlas of Clinical Hematology
They are also a feature of uremia and hepatic coma, where large numbers of these cells are considered a poor prognostic sign. Acanthocyte formation has also been described following treatment with certain drugs. 7. Sickle cells (drepanocytes). Sickle-shaped erythrocytes may occur spontaneously, but sickling is consistently induced by oxygen extraction in the sickle cell test (see p. 9). It signifies a common hemoglobinopathy, Hb S disease (sickle cell anemia), which affects blacks almost exclusively.
23 ' 11' , ,' 24 47 Fig. 7. The neutrophils: myelocytes, metamyelocytes, band and segmented forms -1250 x The offspring of the promyelocytes are the myelocytes (cells 1-13). Generally these cells are somewhat smaller than their immediate precursors, with diameters ranging from 14 to 20 ~. The coarse promyelocytic granules disappear and are replaced by the typical fine neutrophilic or coarse basophilic or eosinophilic (see Fig. 9) granules. The basophilic cytoplasm lightens from the nucleus outward, becoming acidophilic, while the nuclear chromatin becomes coarser.
Incubate each well with 10 III of the respective primary antibody solution for 30 (to 45) min at 20° C (incubation overnight at 4° C is recommended for demonstrating nuclear TdT with polyclonal antibodies). After incubation, first wash each well twice individually (water jet pump) and then submerge slide 10 times in 2 beakers containing PBS. 5. Incubate each well sequentially with 10 III of peroxidase-labeled goat anti-mouse or antirabbit immunoglobulin antibody solution as second layer, and of swine anti-goat immunoglobulin antibody solution as third layer for 30 min each at 20° C.